Seminar by Dr. Prabhat Tripathi, (Northeastern University, USA)
Event Date: 
Wednesday, 3 August 2022 - 6:00pm

Title: Determining the free energy landscape of individual proteins using solid-state nanopore

Speaker: Dr. Prabhat Tripathi

Affiliation: Department of Physics, Northeastern University

Abstract: Protein conformational dynamics have been conventionally studied as a function of variables such as concentration of chemical denaturants, temperature, and pressure. An external electric field can be another perturbation parameter to control protein folding-unfolding dynamics. In this talk, I will present our recently developed method to electrically control the conformational occupancy of the individual proteins during their translocation through a solid-state nanopore. This method enables a new way to quantitatively explore the number of metastable/intermediate states, free energy gaps between each state, short-lived hidden conformational sub-states, transition-path times between states, and protein mutant identification. The method requires only a localized electric field at the solid-state nanopore without any additional requirements such as enzymes, oligo tags, or chemical denaturants.

Venue: 
Zoom
IIT Bombay, Powai, Mumbai